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Characterization of the plant cell wall proteome using high-throughput screens.

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Abstract

Plant cell wall proteins play essential roles in many important biological processes, and yet there is still not a comprehensive catalogue of the cell wall proteome, or "secretome". Here, we describe three procedures, including a yeast secretion trap (YST), Agrobacterium-mediated transient expression using a necrosis-inducing protein (NIP) and protein localization assay using a fluorescent protein, to identify and confirm the localization of cell wall proteins. The approaches are orthogonal and collectively provide a powerful suite of approaches to complement more commonly used strategies to isolate plant cell wall-associated proteins.

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Lee, S. J., & Rose, J. K. C. (2011). Characterization of the plant cell wall proteome using high-throughput screens. Methods in Molecular Biology (Clifton, N.J.), 715, 255–272. https://doi.org/10.1007/978-1-61779-008-9_18

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