Blastocystis Hominis infection among hospitalized children due to diarrhea in Hajar Hospital, Shahre-Kord, Iran

Abstract

Background: Blastocystis hominisis is an intestinal protozoan, recently known as a potential pathogen, which causes intestinal disorders, especially in children. The parasite has a worldwide distribution, ranged from 2% up to 30% in some regions. Objectives: Given no specific study focused on infection rate of B. hominis in children with diarrhea in Iran, this study was carried out to detect frequency of the parasite in children with diarrhea. Patients and Methods: In a cross-sectional study from September 2009 to July 2010, one hundred and sixty hospitalized children with diarrhea in Hajar Hospital, Shahre-Kord, Iran were enrolled. Stool samples were collected and were examined microscopically in wet direct smear using normal saline and iodine solution (Lugol). Furthermore, permanent trichrome stain was performed and all stools were stained and studied with oil immersion's lens (100 ×). Results: Out of 160 stool samples were examined, in 37 cases (23%) including 25 boys and 12 girls, B. hominis was the most frequent protozoa, followed by Giardia lamblia in 19 cases (12%). E. histolitica/dispare were detected only in two cases (1%). Conclusions: The frequency of B. hominis in the study was more than other reports from Iran. This study revealed that among hospitalized children with diarrhea, B. hominis was the most common protozoon. © 2012, Shahid Beheshti University of Medical Sciencces.