Influence of detergent and its components on metabolism of Fusarium oxysporum in submerged fermentation

Abstract

The influence of detergent and its components (sodium tripolyphosphate and ethoxylated cetyl oleyl alcohol) at 0.1 % concentration on the enzymatic and metabolic activity of Fusarium oxysporum during exponential growth was investigated in this paper. The fungus Fusarium oxysporum was isolated from wastewater originating from households which contain detergent. The following biochemical parameters were analyzed: pH, redox potential, proteolytic activity, production of carbohydrates, free and total organic acids, proteins and total dry weight biomass. The detergent had influence on the significant decreasing of redox potential, slight increasing of pH and quantity of glucose and total organic acids, while the proteolytic activity was triple insensive in relation to control. The sodium tripolyphosphate had influence on the slight decreasing of pH, significant increasing of redox potential and quantity of glucose and free and total organic acids, whereas the proteolytic activity was intsensive only 5th and 6th day. The total dry weight biomass of the fungus F. oxysporum was slightly inhibited by ethoxylated alcohol but significantly inhibited by detergent and sodium tripolyphosphate.