Effects of chinaberry tree extract on proliferation, apoptosis, migration and cell cycle distribution of human breast cancer cells

Abstract

To study the effects of chinaberry tree extract on proliferation, apoptosis, migration and cell cycle distribution of human breast cancer cells, the MCF-7 breast cancer cells obtained were divided into blank group, low concentration group, medium concentration group and high concentration group. Phosphate buffered saline solution and sterile physiological saline were added to the blank group for cell culture. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry were used to detect cell proliferation and apoptosis at different time periods. Transwell assay was used to detect cell migration; flow cytometry was used to detect cell cycle distribution, and intercellular cell adhesion molecule-1, matrix metalloproteinase-2, matrix metalloproteinase-9 were detected by Western blotting. The proliferation rate of MCF-7 breast cancer cells in the low, medium and high concentration group was significantly lower than that in the blank group at different time periods, while the apoptosis rate was significantly higher than that in the blank group, and the effect was more obvious with the increase of concentration (p<0.05). The number of cell migration of MCF-7 breast cancer cells in the low, medium and high concentration group was significantly reduced compared with the blank group, and the cell migration was significantly reduced with the increase of the extract concentration of chinaberry tree (p<0.05). The higher the extract concentration of chinaberry tree, the lower the proportion of MCF-7 cells in G0/G1 and S cycle and the higher the proportion of MCF-7 cells in G2/M cycle (p<0.05). The expressions of intercellular cell adhesion molecule-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 in the low, medium and high concentration groups were significantly lower than those in the blank group, and the expressions of intercellular cell adhesion molecule-1, matrix metalloproteinase-2 and matrix metalloproteinase-9 in the high concentration group were significantly lower than those in the low and medium concentration groups (p<0.05). The extract of chinaberry tree can effectively inhibit the proliferation and migration promote the apoptosis and reduce the growth of breast cancer cells by changing the periodic distribution of cancer cells, and its ability is concentration dependent.