Mutations in the p53 tumor suppressor gene in colorectal cancer in Taiwan

9Citations
Citations of this article
13Readers
Mendeley users who have this article in their library.

Abstract

Mutations in the tumor suppressor gene p53 have been reported as occurring prevalently in a wide range of human tumors. Detection of a mutated p53 is thought to provide useful information for the clinical management of colorectal neoplasm. In this study, we used polymerase chain reaction/single-strand conformation polymorphism (PCR/SSCP) and sequencing analysis to rapidly screen for mutations in p53 in colorectal cancer in Taiwan. Genomic DNA was purified from colorectal cancer specimens obtained from 80 patients at a teaching hospital in southern Taiwan. Primer sets were designed to amplify fragments within exons 4-8 of p53. We found p53 mutations in 38 of 80 patients. This is the first identification of a mutation at codon 143 of p53 in colorectal cancer in Taiwan. In addition, we found two insertions in exon 5 of p53. The p53 mutation rate among colorectal tumors in Taiwan, found in this study, is 43%. The results indicate that p53 mutation is not significantly associated with tumor grade, age, or gender (p > 0.05). We found that two-fifths of colorectal cancer patients in Taiwan have a p53 mutation, which could be used as a marker of colorectal cancer.

Cite

CITATION STYLE

APA

Chang, F. H., Tzeng, D. S., Lee, T. M., Chen, T. C., Hsu, L. S., & Lung, F. W. (2003). Mutations in the p53 tumor suppressor gene in colorectal cancer in Taiwan. Kaohsiung Journal of Medical Sciences, 19(4), 151–157. https://doi.org/10.1016/s1607-551x(09)70464-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free