The effects of progesterone, dehydroepiandrosterone sulfate and 17β-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium. The changes in the radioactivity of hyaluronate showed that progesterone suppressed hyaluronate synthesis by 22% of the control levels, while dehydroepiandrosterone sulfate and 17β-estradiol enhanced it by 22% and 12% of the control levels, respectively. Furthermore, progesterone induced degradation of high-molecular-weight [14C]hyaluronate into low-molecular-weight hyaluronate (M(r) ~40,000). These results suggest that in cultured fibroblasts from the human uterine cervix progesterone converts hyaluronate metabolism from the synthesis phase to the degradation phase.
Tanaka, K., Nakamura, T., Takagaki, K., Funahashi, M., Saito, Y., & Endo, M. (1997). Regulation of hyaluronate metabolism by progesterone in cultured fibroblasts from the human uterine cervix. FEBS Letters, 402(2–3), 223–226. https://doi.org/10.1016/S0014-5793(96)01538-4