Effects of Candida albicans infection on defense effector secretion by human oral mucosal epithelial cells

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Abstract

Objective: The aim of this study was to investigate the effects of Candida albicans on the production of defense effector molecules by human oral mucosal epithelial cells in vitro. Design: Immortalized human oral mucosal epithelial (Leuk-1) cells and C. albicans strain 5314 were cocultured at different cell-to-C. albicans ratios. The viability of Leuk-1 cells was determined by MTT and RTCA measurements. The secretory levels of multiple defense effector molecules were determined by Enzyme-linked immunosorbent assay (ELISA). Results: Our results indicated that C. albicans significantly decreased the secretion of IgG, cystatin C, lactoferrin, and TGF-β1 in a dose-dependent manner and remarkably reduced the production of IgA independent of the cell-to-C. albicans ratio. However, C. albicans clearly enhanced the secretion of IgM, galectin-3, P-selectin, granzyme B and perforin. Conclusion: These results suggest that C. albicans may exert a regulatory role in the defense response of oral mucosal epithelial cells by altering secretory levels of defense effector molecules.

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Huang, F., Song, Y., Chen, W., Liu, Q., Wang, Q., Liu, W., … Wang, W. (2019). Effects of Candida albicans infection on defense effector secretion by human oral mucosal epithelial cells. Archives of Oral Biology, 103, 55–61. https://doi.org/10.1016/j.archoralbio.2019.05.013

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