From thioesters to amides and back: Condensation domain reversibility in the biosynthesis of vibriobactin

Abstract

(Chemical Equation Presented) Reloaded. In the biosynthesis of vibriobactin, the two condensation domains responsible for catalyzing amide bond formation between three catechol-containing moieties and norspermidine (NS in scheme) to yield the mature siderophore, were found to be reversible, and both were capable of reloading their cognate thiolation domains with the acyl components of their amide products. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.