Ameliorating effect of Celastrus paniculatus standardized extract and its fractions on 3-nitropropionic acid induced neuronal damage in rats: Possible antioxidant mechanism

Abstract

Context: Celastrus paniculatus Wild. (Celasteraceae) (CP) is a well-known Ayurvedic ‘Medhya Rasayana’ (nervine tonic), used extensively as a neuro-protective and memory enhancer, and in different central nervous system disorders. Objective: To evaluate the effect of CP against 3-nitropropionic acid (3-NP) induced Huntington’s disease (HD) like symptoms in Wistar male rats. Materials and methods: The ethanol extract of CP seeds (CPEE), prepared by maceration, was standardized on the basis of linoleic acid content (6.42%) using thin layer chromatography densitometric analysis. Protective effect of CPEE (100 and 200mg/kg) and its various fractions, viz., petroleum ether (40 mg/kg), ethyl acetate (2.5 mg/kg), n-butanol (7 mg/kg) and aqueous (18mg/kg), administered orally for 20 days, against 3-NP (10mg/kg, i.p. for 14 days) was assessed by their effect on body weight, locomotor activity, grip strength, gait pattern and cognitive dysfunction and biochemical parameters for oxidative damage in the striatum and cortex regions of the brain. Results: CPEE (100 and 200 mg/kg) treated animals exhibited a significant (p<0.05) improvement in behavioural and oxidative stress parameters in comparison to only 3-NP treated animals. Amongst various tested fractions of CPEE, aqueous fraction (AF) at 18mg/kg exhibited maximum reversal of 3-NP induced behavioural and biochemical alterations, and was therefore also tested at 9 and 36mg/kg. CPEE (100mg/ kg) and AF (36mg/kg) exhibited maximum and significant (p<0.05) attenuation of 3-NP induced alterations in comparison to 3-NP treated rats. Conclusions: CPEE has a protective action against 3-NP induced HD like symptoms due to its strong antioxidant effect.