Atomic force microscopic observation of Escherichia coli ribosomes in solution

Abstract

Ribosomes of Escherichia coli were visualized in buffer solution by atomic force microscopy (AFM). A series of time-dependent AFM images showed that ribosomes spontaneously adsorb on mica. Although ribosomes observed in air are forced to flatten on the surface, the height of ribosomal particles obtained under a physiological buffer solution is 21.8±0.5nm, which is consistent with the ideal diameter. We succeeded in observing single ribosomes in a near-native condition.