Antibacterial activity test combination of Indigofera zollingeriana and turmeric (Curcuma domestica val.) as alternative Feed Additive for poultry

Abstract

Indigofera zollingeriana and turmeric are potentially antibacterial plants. Tannin and phenol in Indigofera zollingeriana and turmeric are able to block the formation of bacterial cells and lyse cell membranes. The purpose of this research was to examine the antibacterial effectiveness contained in Indigofera zollingeriana and turmeric flour against Salmonella sp., Escherichia coli and Lactobacillus sp. The research consisted of 5 treatments and 4 replications with T0 treatment: Tetracycline (Control), T1: Indigofera zollingeriana 5% T2: Indigofera zollingeriana 10%, T3: Indigofera zollingeriana 15%, T4: Indigofera zollingeriana 20% and addition of turmeric powder as much as 2.5% at the level of T1, T2, T3 and T4. The research designed according to the Completely Randomized Design and Duncan test was performed to defining the difference among treatments. The results showed that the inhibition zone around the wells of Escherichia coli bacteria was T0: 33.03, T1: 11.02, T2: 11.42, T3: 11.72, T4: 11.32; Salmonella sp. T0: 24.12, T1: 10.68, T2: 12.33, T3: 14.12, T4: 13.65 and Lactobacillus sp. T0: 33.61, T1: 25.67, T2: 25.93, T3: 26.65, T4: 27.16 mm, respectively. It was concluded that the inhibition zone was influenced by several factors such as the concentration of samples used and the properties of bacteria. The combination of Indigofera zollingeriana and turmeric can be used as an alternative feed additive for poultry.