Aseptic cultivation of Coprinus comatus (O. F. Mull.) Gray on various pulp and paper wastes

Abstract

The chemical components of pulp and paper wastes from industrial paper mill were analyzed prior to aseptic cultivation. Mycelial running, primordium initiation, fruiting body development, yield and bioefficiency of Coprinus comatus on pulp and paper waste supplemented with rice bran were studied. Chemical analysis revealed that brown paper waste contains 48 ppm of Pb. The fastest mycelia colonization (8 days), primordium initiation (12 days) and fruiting body development (14 days) were realized in substrate composed of pure coarse gray paper waste. However, the highest yield (9.53 g) and biological efficiency (23.96%) were recorded in the formulation containing light blue paper waste + 10% rice bran. The fruiting bodies produced in contaminated paper wastes were detected to consist of 16.15 ppm of Pb. In general, we have successfully demonstrated the cultivation of C. comatus on pulp and paper wastes enriched with rice bran and its ability to absorb Pb from contaminated substrates.