Preclinical Evaluation of Efficacy of Processed PRP and Fresh PRP in Diabetic Wound Healing

Abstract

Background: One in four people with diabetes will experience diabetic wounds at some point in their lifespan, which is among the diabetes complications that have the worst effects on quality of life. The study’s objective was a comparative preclinical study of the efficacy of fresh platelet-rich plasma (PRP) Vs L-PRP in diabetic wound. Methods: Twenty four rabbits were used to study the efficacy. Diabetes was generated in the rabbits, and the diabetic wound’s perilesional region received PRP treatment. The comparative evaluation by done by counting the wound area and rate of healing. Results: There was more than three folds rise in growth factors in lyophillised-PRP than compared to fresh PRP. The rate of wound healing was much fast in lyophilised PRP group. In the control group the wound was unhealed by 30th day and also showed pus cell formation and symptoms of infection. However, it was completely healed on 25th day when treated with L-PRP stored at 8℃. Conclusion: The outcomes prompted clinical research to compare L-PRP to fresh PRP’s efficacy.