Localization of α-tocopherol synthesis in chromoplast envelope membranes of Capsicum annuum L. fruits

Abstract

Chromoplast membranes from Capsicum fruits were fractionated by isopycnic continuous sucrose gradients resulting in the separation of outer (d=1.08 g cm-3) and inner (d=1.11 g cm-3) envelopes. Both membranes were characterized by marker enzymes and with antibodies against the phosphate translocator. This report is concerned with the location of α-tocopherol synthesis in these membranes.