Protein-phosphorylation-responsive polymeric nanoparticles for imaging protein kinase activities in single living cells

Abstract

(Figure Presented) Take your PIC: Cell-permeable and biocompatible polyion-induced complex (PIC) nanoparticles were developed consisting of a negatively charged polymer along with a positively charged polymer conjugated to a near-infrared fluorophore and a protein-kinase-specific peptide. Owing to profound changes in fluorescence, the PIC nanoparticle is an attractive probe for protein kinase A (PKA) in single living cells. ATP = adenosine triphosphate, ADP = adenosine diphosphate. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.