Spirosoma rigui sp. nov., isolated from fresh water

Abstract

A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB118T, was isolated from fresh water collected from the Woopo wetland (Republic of Korea). Cells were rod-shaped and sometimes filamentous. The major fatty acids were iso-C15:0 2-OH and/or C16:1ω7c (45.6%), C16:1ω5c (18.5%), iso-C15:0 (9.5%) and C16:0 (8.8%). The predominant menaquinone and polar lipid were MK-7 and phosphatidylethanolamine, respectively. The DNA G + C content was 53.3 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB118T had an evolutionary lineage within the radiation encompassing the members of the family 'Flexibacteraceae', its closest neighbour being Spirosoma linguale LMG 10896T (93.7 % gene sequence similarity). Data from this polyphasic study indicated that strain WPCB118T could not be assigned to any recognized species. Strain WPCB118T represents a novel species of the genus Spirosoma, for which the name Spirosoma rigui sp. nov. is proposed. The type strain is WPCB118T (=KCTC 12531T=NBRC 101117T). © 2007 IUMS.