Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB)

Abstract

Centella asiatica (L.) Urb. known as Brahmi, Indian Pennywort and Mandookaparni is a small herbaceous annual plant of the family Apiaceae, It is used in traditional medicine for the treatment of various ailments & ayurvedic treatment for many diseases. Present study deals with the qualitative and quantitative analysis of ethanolic extract of root, stem & leaf of Centella asiatica (L.) Urb. A qualitative analysis by thin layer chromatography & a quantitative analysis by standard chemical protocol of secondary metabolites in the root, stem and leaf of the Centella asiatica L. (URB) have been studied. Using thin layer chromatography (TLC) different components like Alkaloids, Saponin, Flavonoids, Terpenoides, Phenol & Tannin are isolated & identified. The Rf values of the developed spots in the different solvent systems are noted. In the quantitative analysis, alkaloids, saponins, terpenoids & flavonoids are extracted by using the standard chemical protocol. These results may be helpful for rationale use of this plant in the modern system of health care.