Electrophoretic profile of hybrids between cryotolerant and non-cryotolerant Saccharomyces strains

Abstract

The chromosomal DNAs of cryotolerant Saccharomyces bayanus, non-cryotolerant Saccharomyces cerevisiae strains and their intra and interspecific hybrids were separated by pulsed field electrophoresis (PFGE). The cryotolerant and non-cryotolerant strains gave distinctly different electrophoretic profiles. The hybrids cryotolerant x cryotolerant and non-cryotolerant x non-cryotolerant were fertile and they gave the same electrophoretic karyotype as the respective parents. The cryotolerant x non-cryotolerant hybrids were sterile and gave electrophoretic karyotypes which showed both the bands the parents have in common and those they do not share.