Characterization and antimicrobial activity of a halophyte from the asturian coast (Spain): Limonium binervosum (g.e.sm.) c.e.salmon

Abstract

The work presented herein deals with the characterization and valorization of a halophyte from the cliffs of the Asturian coast: Limonium binervosum (G.E.Sm.) C.E.Salmon (rock sea-lavender). Its biomass and hydromethanolic extracts were studied by elemental and thermal analysis, infrared spectroscopy and gas chromatography–mass spectroscopy. Tetradecanoic acid/esters and 1,2-tetradecanediol were identified in its flower extract, while the leaf extract was rich in linolenic and linoleic acids and their esters, hexadecanoic acid and its esters, and phytol. Both flower and leaf hydromethanolic extracts contained eicosane, sitosterol and tocopherols in significant amounts. With a view to its valorization, the antimicrobial activity of these extracts was investigated against three apple tree and grapevine phytopathogens. Both the hydroalcoholic extracts and their main constituents, alone or in combination with chitosan oligomers (COS), were tested in vitro. A remark-able antibacterial activity was observed for the conjugated complexes of the flower extract with COS, both against Xylophilus ampelinus (MIC = 250 µg·mL−1 ) and Erwinia amylovora (MIC = 500 µg·mL−1 ), and complete inhibition of the mycelial growth of Diplodia seriata was found at concentrations <1000 µg·mL−1 . In view of these results, this extremophile plant can be put forward as a promising source of bioactive metabolites.