Polyphenolic determination from medicinal plants used in veterinary medicine by an UHPLC-LC-MS/MS method

Abstract

Flavonoids and polyphenols are known for positive health effects and are used for their medicinal properties. They are considered functional foods and are widely distributed in plants having an important role in health benefits. In this study, several phenolic compounds have been determined quantitatively from eighteen medicinal plants. Chromatographic separation of analytes was performed using a NUCLEODUR C18 Gravity, 3 µm, 150 x 3 mm column using a mobile phase consisting of 0.2% formic acid in water and methanol with gradient elution and a flow rate of 0.6 mL/min. Detection was performed in multiple reaction monitoring (SRM) mode for chlorogenic acid, caffeic acid, quercitrin, luteolin, quercetin, apigenin, and kaempferol. Plant extracts with methanol and ethanol (70⁰) were prepared. The studied medicinal plants revealed richness in flavonoids and polyphenols. The present study serves as a contribution to the characterization of phenols and flavonoids from medicinal plants traditionally used in human and veterinary medicine.