DEVELOPMENT OF IN VITRO PLANT REGENERATION PROTOCOL OF BANANA (MUSA SP.) CV. GRAND NAINE USING SUCKER EXPLANT

Abstract

Banana is an important fruit crop belongs to the family Musaceae. This has more demand for it multifarious uses like food, medicinal as well as industrial values. The present study was carried out to develop micropropagation protocol for large scale production of banana cv. Grand naine using sucker explant. Sucker explants were inoculated on Murashige and Skoog’s (1962) (MS) basal medium and MS basal medium supplemented with different types and concentrations and combination of plant growth regulators. Highest mean number of shoots (10.2) per explant having mean shoot length 5.2 cm was observed on MS medium supplemented with 4.0 mg/L BA, 2.0 mg/L Z, 1.0 mg/L NAA, and 3.0 mg/L ADS. For large scale production of shoot, in vitro regenerated shoots were harvested, cut into small pieces and inoculated on the optimum medium for multiple shoot proliferation. In this way, more than thousand numbers of in vitro shoots were regenerated from a single explant at six month of culture. In vitro regenerated shoots were excised and rooted on ½ MS medium supplemented with 1.0 mg/L IBA. Finally in vitro regenerated plants were acclimatized and subsequently transferred to field with zero mortality. This protocol helps to meet the demand of the farmers.