In vitro anti-bacterial screening of drynariya quercifolia

Abstract

Purpose: To make scientific validation with antibacterial activity of ethnomedical fern Drynaria quercifolia Method and results: The various rhizome extracts were prepared with different solvents   like benzene, Ethyl acetate, chloroform, Ethanol, water and their percentage yield were identified as 7,3,2.3,3.1&6.4 respectively and analytical parameters and histological studies undergone with this fern. Various preliminary phytoconstituent screening was carried out and the ethyl acetate extract showed the maximum presence of active constituents. Bacterial activity was screened with (gram-positive and gram-negative bacteria) and the zone of inhibition was measured. Compared with standard Ampicillin (250µgm) and ethyl acetate was more active and most effective against Bacillus subtilis. Conclusion: In the antibacterial study ethyl acetate extract shows good antibacterial activity even in microgram quantities. It is found to be very effective against Bacillus subtilis. From the studies we have concluded that Drynaria quercifolia can be effectively used as an anti-bacterial agent.