Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains

Abstract

Human rotaviruses were isolated directly from stool specimens of gastroenteritis patients in MA-104 cells in the presence of trypsin. For the plaque assay of the isolated strains, the optimal composition of overlay medium was determined. The antigenicity of the isolated strains was investigated by a plaque neutralization method, using antisera prepared against six strains having different electropherotypes of viral RNA, and three different neutralization serotypes were denonstrated.