Effects of Straw Maize on the Bacterial Community and Carbon Stability at Different Soil Depths

Abstract

In order to test the short-term effects of straw amendment on soil organic C (SOC) stabilization, SOC protection mechanisms, and soil bacterial community, we examined which bacterial taxonomic groups were associated with protected C fractions via different soil depths. We conducted a 5-year field experiment including a total of four treatments: S0 (no straw amendment), S1 (0–20 cm straw-amended soil), S2 (0–40 cm straw-amended soil) and S3 (0–60 cm straw-amended soil). The core method was used for soil sampling, and 180 soil samples was collected. Our results showed that straw amendment significantly increased bulk soil C content, enhanced the constituents of physically separated fractions and their OC contents, and changed the soil bacterial community composition at different soil depths. SOC was more accelerated in macroaggregate-derived unprotected and microaggregate-derived physically protected fractions at soil depths of 0–20 cm. Physically protected and physico-biochemically protected fractions were the major C protection mechanisms at soil depths of 20–40 cm and 40–60 cm soil depths. Our study also provides evidence that straw amendment significantly increases the bacterial phyla abundance of Proteobacteria and Bacteroidetes at each soil depth. Moreover, straw amendment enhanced the relative abundances of Gemmatimonadetes and Nitrospirae at soil depths of 40–60 cm and have a positive correlation with physically and physico-biochemically protected C pools. These results indicate that straw amendment can regulate C sequestration processes by enhancing SOC physical protection and modulating bacterial community, especially in the deep soil. In addition, straw amendment in subsoil (0–40 cm or 0–60 cm) is more beneficial for C storage and stabilization.