Optimization of inulinase production using copra waste by K. marxianus var. marxianus

Abstract

Kluyveromyces marxianus var marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett-Burman design. Corn steep liquor, (NH4)2SO4, ZnSO4.7H2O, K2HPO4 and urea were selected based on their positive influence on inulinase production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are: corn steep liquor - 0.0560 (g/gds), (NH4)2SO4 - 0.0084 (g/gds), ZnSO4.7H2O - 0.0254 (g/gds), K2HPO4 - 0.0037 (g/gds) and urea - 0.02147 (g/gds). These conditions were validated experimentally which revealed an enhanced inulinase yield of 372 U/gds.nema