Polymorphisms of codons 110, 146, 211 and 222 at the goat prnp locus and their association with scrapie in greece

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Abstract

Scrapie is considered an endemic disease in both sheep and goats in Greece. How-ever, contrary to sheep, in goats more than one prion protein (PrP) polymorphism has been rec-ognized as a candidate for resistance breeding against the disease. For an impression, candidates which are circulating, (i) brain samples (n = 525) from scrapie-affected (n = 282) and non-affected (n = 243) animals within the national surveillance program, and (ii) individual blood samples (n = 1708) from affected (n = 241) and non-affected (n = 1467) herds, in a large part of mainland Greece and its islands, were collected and assayed. A dedicated Taqman method was used to test for amino acid polymorphisms 110T/P, 146N/S/D, 211R/Q, and 222Q/K. Highly prevalent genotypes were 110TT, 146NN, 211RR, and 222QQ. The frequencies of polymorphisms in blood and negative brain samples for codons 110P, 211Q, and 222K were 4.0%, 3.0%, and 1.9%, respectively, while 146D (0.7%) was present only on Karpathos island. Codon 110P was exclusively found in scrapie-negative brains, and homozygous 110P/P in two scrapie-negative goats. It is concluded that breeding programs in Karpathos could focus on codon 146D, while in other regions carriers of the 110P and 222K allele should be sought. Case-control and challenge studies are now necessary to elucidate the most efficient breeding strategies.

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Gelasakis, A. I., Boukouvala, E., Babetsa, M., Katharopoulos, E., Palaska, V., Papakostaki, D., … Ekateriniadou, L. V. (2021). Polymorphisms of codons 110, 146, 211 and 222 at the goat prnp locus and their association with scrapie in greece. Animals, 11(8). https://doi.org/10.3390/ani11082340

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