Functional molecular size of trypsin inhibitors as determined by radiation inactivation analysis

Abstract

The molecular sizes of trypsin inhibitors obtained by radiation inactivation were investigated in relation to the functional domain size. The molecular weights obtained were 10 200 ± 700 for ovomucoid, 17 800 ± 400 for ovoinhibitor and 16 400 ± 500 for soybean trypsin inhibitor. These values mostly agreed with the size of domain which has trypsin inhibitory activity, suggesting that the radiation inactivation analysis indicates the minimum functional domain size. © 1987.