Panton–valentíne leukocidin genes in a phage-like particle isolated from mitomycin C-Treated staphylococcus aureus V8 (ATCC 49775)

Abstract

The staphylococcal Panton–Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (ϕPVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in ϕPVL was a linear double-stranded 40-kb DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase géne) of ϕPVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus. © 1997, Taylor & Francis Group, LLC. All rights reserved.