Determination of flufenacet and its metabolites in agricultural products by LC-MS/MS

Abstract

A simultaneous analytical method based on LC-MS/MS was developed for the determination of flufenacet and its metabolites, ［（4-fluorophenyl（）1-methylethyl）amino］oxo-acetic acid and ［N-（4-fluorophenyl）-N（- 1-methylethyl）acetamide］-2-sulfinylacetic acid, in agricultural products. The compounds were extracted from samples with methanol. The crude extracts were purified using Bond Elut C18 and InertSep GC/PSA, then determined by LC-MS/MS. The average recoveries（n＝5）from 4 kinds of agricultural products（wheat, soybean, potato and tomato）spiked at the level of the MRLs or the uniform limits（0.01 μg/g）were 70.6–97.0％, and the relative standard deviations were less than 5％. The lower limits of quantitation of flufenacet and its metabolites were 0.01 μg/g.