Liquid chromatography with hydrocarbonaceous bonded phases; theory and practice of reversed phase chromatography

Abstract

The use of nonpolar bonded phases in high-performance liquid chromatography is reviewed. The properties of the most commonly employed stationary phases and eluents are discussed. A concise theoretical treatment of the interaction between solutes and hydrocarbonaceous bonded stationary phases is used to shed light on the major factors which govern solute retention in reversed phase chromatography. Recent applications of the technique to the separation and microanalysis of a wide variety of substances are illustrated by numerous references. The fundamental aspects of ion-pair reversed phase chromatography are discussed. The potential use of nonpolar stationary phases for physico-chemical measurements by HPLC is pointed out. © 1977 Oxford University Press.