Reverse transcription combined with the polymerase chain reaction (RT-PCR) is a viable method widely used to quantify gene expression. There are two ways to quantify gene expression by real-time PCR: relative quantification and absolute quantification. Relative quantification relates the PCR signal of the target gene to a control gene, normally a housekeeping gene. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Here we describe both methods from RNA extraction to its quantification by real-time PCR.
CITATION STYLE
Mazza, R., & Mazzette, R. (2014). Absolute and relative gene expression in listeria monocytogenes using real-time PCR. Methods in Molecular Biology, 1157, 213–221. https://doi.org/10.1007/978-1-4939-0703-8_18
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