A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrations

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Abstract

Experiments were performed to optimize the macronutrients concentrations for in vitro rooting of Ceratonia siliqua micropropagated shoots. Several dilutions of Murashige and Skoog (MS) medium were tested: full-strength MS, half-strength MS (1/2MS), and 1/2MS + full N. The frequency of in vitro rooting was enhanced when the 1/2MS was used (50 % rooted shoots). Mature leaves from 20 - 30 year-old carob trees and from 2 year-old micropropagated plants were collected and the concentrations of macronutrients (N, P, K, Ca, Mg) assessed. Based on the mineral composition of the leaves a new medium was formulated and compared with the previous ones showing an increment of the rooting frequency to 80 %. Moreover, shoots rooted in the new medium did not show symptoms of apical necrosis that occurred in the other tested media.

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Gonçalves, S., Correia, P. J., Martins-Loução, M. A., & Romano, A. (2005). A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrations. Biologia Plantarum, 49(2), 277–280. https://doi.org/10.1007/s10535-005-7280-4

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