Regulation of λ rex Expression After Infection of Escherichia coli K by Lambda Bacteriophage

  • Astrachan L
  • Miller J
16Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The ability of Escherichia coli K to support bacteriophage T4rII replication starts to decline at 3 to 6 min after infection by λ. This inhibition appears to depend on expression of the λ rex + gene, since little inhibition was observed following infection by a λ rex mutant or by a hybrid bacteriophage λi 434 which lacks a functional rex gene. For promotion of the synthesis of rex product, cII + and N + genes are required and can act trans , whereas cY + , also required, must be cis to a rex + gene. These genes presumably play a role in the transcription of the cI- rex operon because they are also known to be required for repressor (cI product) synthesis. Functional cIII, O, P genes are not necessary for ample rex product synthesis. We also observed full rex expression after infection by λ sus cI mutants, suggesting that rex and repressor are separate gene products and that repressor is not required for inhibition of T4rII replication. We also report experiments with a rex mutant that is not leaky when in a lysogen but is sufficiently leaky shortly after infection to cause inhibition of T4rII replication.

Cite

CITATION STYLE

APA

Astrachan, L., & Miller, J. F. (1972). Regulation of λ rex Expression After Infection of Escherichia coli K by Lambda Bacteriophage. Journal of Virology, 9(3), 510–518. https://doi.org/10.1128/jvi.9.3.510-518.1972

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free