The male gametophyte (or pollen) is a highly specialized organ essential for sexual reproduction of higher plants. Their reduced complexity constitutes them as an ideal experimental system for analyses of biological processes maintaining tip growth. Rapid advances in proteomic technologies and a vast choice of metabolic labelling and label-free quantitation protocols as well as the availability of full genome sequences allow comprehensive analyses of various pollen proteomes. Pollen membrane proteome consists of integral and membrane-associated proteins involved in regulation of many cellular functions. In this chapter, novel insights into identification of membrane proteins by proteome analysis and how their dynamic subcellular localization contributes to the initiation of pollen grain germination and maintenance of tube growth are discussed.
Pertl-Obermeyer, H. (2017). The pollen membrane proteome. In Pollen Tip Growth: From Biophysical Aspects to Systems Biology (pp. 293–318). Springer International Publishing. https://doi.org/10.1007/978-3-319-56645-0_11