UJI AKTIVITAS ENZIM PENISLIN G ASILASE (PGA) SECARA KUALITATIF TERHADAP ISOLAT Escherichia coli YANG BERASAL DARI AIR SUNGAI DI KOTA PADANG

Abstract

Penicillin G acylase is catalysed hydrolisis enzym penicillin G to 6- amino penicilanad acid (6-APA). 6-APA is precursor to made semisinthetic penicillin. Semisynthetic penicillin derivated of natural penicillin that get it by change natural penicillin chemistry structure or sinthesis nuclear penicillin. Penicillin G acylase (PGA) produce of bacteria but bacteria produced number penicillin is limited so looking new bacteria penicillin produce is importen to do. Based it haved bacteria isolation in Padang town any rivers, include air batang Kandis, air batang Kurao, air batang Kuranji, air batang Arau dan air batang Aru di kota Padang. This research do in Kopertis wilayah X laboratory. Activity assay PGA used Meevootisom methode is metode easy to selection, rapid and spesifik to selection bacteria strain PGA produced. Meevootisoom metode used Serratia marsescens, is resisten to penicillin and sensitiv to 6-APA. Result of this research is every isolated from all rivers contain PGA.