Regulation of human MutYH DNA glycosylase by the E3 ubiquitin ligase mule

Abstract

Background: Regulation of MutYH DNA glycosylase is essential for genomic integrity. Results: MutYH is ubiquitinated by the E3 ligase Mule changing its protein levels and recruitment to chromatin. MutYH levels in cancer cells influence the mutagenic potential of reactive oxygen species. Conclusion: Ubiquitination of MutYH is an important regulatory step. Significance: We identified the residues that are targets for ubiquitination by Mule. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.