Identification of 1BL/1RS Translocation Based on Mixograph Parameters in Common Wheat

Abstract

Abstract：lBL／lRS translocation has been widely used for improving agronomic performance and disease resistance in wheat (Triticum aestivum L．)，however,it has strong negative effect on processing qualitv．Tb develop a method for 1BUlRS translocation identification with mixograph parameters．404 advanced lines from 146 crosses in 2005—2006(experiment I)and 175 ad． vanced lines and main cultivars of Shandong province(experiment II)in 2005-2006 and 2006_2007 cropping seasons were used in this study．A11 materials were sown under irrigation condition in a randomized complete design with 1 replication in Jinan．The genetic effect of lBL／lRS translocation on mixograph parameters was investigated．The variations of mixograph parameterS under different combinations of the high molecular weight glutenin subunits(HMW-GSl and low molecular weight glutenin subunits (LMW-GSl were also analyzed．1 BL／1 RS translocation lines showed significantly shorter mixing time，less bandwidth of peak and bandwidth after 1 min peak．and higher angle of descent and the bandwidth ratio(the ratio of bandwidth of peak／bandwidth after l min peak)in comparison with non．1BL／1RS translocation lines．It indicated that the lBL／lRS translocation has deleterious effects on mixograph parameters．Mixograph of the lBL／J RS translocation was characterized with the bandwidth sharply declin． ing and narrowing after l min peak．and increasing the bandwidth ratio．whereas the bandwidth of non—lBL／lRS translocations declined gently after l min peak or had a longer mixing tolerance．and had a little variation about the bandwidth ratio．Furthermore， 85．2％(experiment I)and 96．8％(experimentⅡ)accuracies were achieved in grouping the lBL／lRS translocation and non．1BL，1RStranslocation onthebasisofthebandwidth ratio，i⋯e 1BL／1RStranslocationline had avaluemorethan orequaltO 1．6．and non．1BL／lRS translocation line had a valHe smaller than 1．6．Although the Glu—B3 alleles showed better quality parameters when HMW．GS 5+l 0 was presented．it was still the most anfavorable allele on mixograph parameters among all Glu—B了小 leles．Therefore，mixograph parameters could be used tO determine the rheological properties and the presence of 1BL／1RS trans— location．