Quantitative Analysis of Andrographolide in Sambiloto (Andrographis Paniculata) Herbal Preparation Using UV-Vis, HPLC, And 1H-NMR With Dibenzyl Ether as Internal Standard

Abstract

The therapeutic potential of Sambiloto (Andrographis paniculata), particularly its bioactive compounds with antiviral and anti-inflammatory properties, has led to increased demand. This study aimed to quantify the Andrographolide content in Sambiloto herbal preparations using UV-Vis Spectrophotometry, HPLC, and qHNMR, with dibenzyl ether as an internal standard. The UV-Vis analysis showed Andrographolide content of 2.68% and 2.39% using external and internal standards, respectively. HPLC analysis, under optimal conditions (Acetonitrile: 0.1% o-phosphate, 40:60), revealed a content of 0.1925%. qHNMR analysis, using dibenzyl ether, indicated a content of 0.233%. Dibenzyl ether demonstrated potential as an innovative internal standard, offering a cost-effective alternative to expensive pure standards in qHNMR. This study enhances cost-effective and accurate methods for quantifying bioactive compounds in herbal preparations.