High pressure liquid chromatographic determination of bergenin and (+) -afzelechin from different parts of Paashaanbhed (Bergenia ligulata Yeo)

Abstract

A reverse-phase high pressure liquid chromatographic method with an optimised extraction procedure for the quantitative estimation of bergenin and (+)-afzelechin in different parts of the popular Indian herb Paashaanbhed (Bergenia ligulata Yeo) has been developed. The method is sensitive, precise and gives more than 99% recovery of the active constituents. The rhizomes of the plant were found to contain higher concentrations of bergenin (0.907% w/w) and (+)-afzelechin (0.168% w/w) than other parts of the plant. The presence of these constituents was also estimated in polyherbal formulations containing this plant.