The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe

Abstract

Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified. Copyright © 2007 by the Genetics Society of America.