Isolation and characterization of plant growth promoting Burkholderia spp

Abstract

In this study, a total of eight Burkholderia isolates obtained from rhizosphere soil of\rmaize, cotton, rice, sugarcane, groundnut and soybean and tested for the N fixation,\rphosphate solubilisation and antagonistic activity against Rhizoctonia solani. All the\reight isolates produced the band for the nifH gene in the polymerase chain reaction\rconfirmed the presence of nifH gene. Among the 8 isolates, RMS1 produced clear halo\rzone around 7 mm on surface of HAP medium. CMS1, RMS1 and SMS1 are inhibited\rthe growth of Rhizoctonia solani for 43, 40 and 35 per cent, respectively. An isolate\rRMS1 possessed all the three plant growth promoting traits viz., higher phosphate\rsolubilizing ability, antagonistic activity and presence of nifH gene selected for molecular\rcharacterization with amplifying and sequencing of 1.46 kb 16S rRNA gene. Based on\rthe BLASTn homology it was found that 16S rRNA sequence of isolate RMS1 having\r93 per cent identity with showed 97 per cent homology with Burkholderia thailandensis.