Whole exome capture in solution with 3 Gbp of data

Abstract

We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies. © 2010 Bainbridge et al.; licensee BioMed Central Ltd.