Continuous-flow analysis for ethanol with immobilized alcohol dehydrogenase

Abstract

Alcohol dehydrogenase is immobilized by reaction with glutaraldehyde onto silica gel, which is packed in a column in a flow system. Ethanol (0.1-5 mmol) is determined by catalysis of the reaction with NAD+, the NADH produced being detected amperometrically. The reproducibility is ca. 1%. © 1982.