Improvement of cell counting method for Neubauer counting chamber

Abstract

Background: We compared the cell counting accuracy of the conventional method and the improved method by using Neubauer counting chamber. Methods: In the improved method, all the border cells were counted and then divided by two; while, in the conventional method, only border cells on the two boundaries (top and left) were counted. Results: About 55.814% of the samples showed more accurate results by improved counting method, about 38.372% had more accurate results by conventional counting method, and about 5.814% were counted with similar counting error by both methods. The improved method had significantly smaller counting error than conventional method (P