Morphological and Immunocytochemical Characterization of Osteoblast Cultures from Long Bones of Neonatal Rats

Abstract

The present study reports a successful culture system of rat bone cells obtained from the bare long bone fragments of neonatal Wistar rats. The morphology, histochemical and immunocytochemical properties of the cultured cells were characterized by phase contrast microscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), histochemical staining with alkaline phosphatase (ALP), and immunocytochemical staining with the anti-osteocalcin antibodies. © 1992, International Society of Histology and Cytology. All rights reserved.