PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases

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Abstract

The replication fidelities of Pfu, Tag, Vent, Deep Vent and UITma DNA polymerases were compared using a PCR-based forward mutation assay. Average error rates (mutation frequency/bp/duplication) increased as follows: Pfu (1.3 x 10-6) < Deep Vent (2.7 x 10-6) < Vent (2.8 x 10-6) < Tag (8.0 x 10-6)

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APA

Cline, J., Braman, J. C., & Hogrefe, H. H. (1996). PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases. Nucleic Acids Research, 24(18), 3546–3551. https://doi.org/10.1093/nar/24.18.3546

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