Separation of neutral lipid classes from bee-collected pollen by high performance liquid chromatography (HPLC)

Abstract

A fast method for the separation of neutral lipid classes of bee-collected pollen by isocratic HPLC is described using ultraviolet detection at 206 nm and direct injection of the sample into a silica column. The mobile phase was n-hexane-2-propanolacetic acid (100:0.5:0.1). Three fractions of neutral lipids were isolated from 35 samples: Sterol esters and other nonpolar compounds such as carotenoids and waxes; triglycerides; and fatty acids.