Nigella sativa seed-mediated green biosynthesis of silver nanoparticles and antimicrobial activity

Abstract

This work introduces a systematic and efficient approach for producing stable AgNPs utilizing Nigella sativa (Ns) seed aqua extract (AE), which exhibit strong antibacterial properties. The characterization of Ns-AgNPs was performed using a UV-visible spectrophotometer (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM), transmission electronic microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). The Ns-AgNPs did not show aggregation, as shown by the results of STEM and XRD. According to the EDX analysis in this research, it was determined that Ns-AgNPs, gave signals in the silver region (~ 3 KeV) at 92.3%. Dynamic light scattering (DLS) was used to determine the average particle size and distribution profile of Ns- AgNPs. Ns-AgNPs showed significant antibacterial performance against Staphylococcus aureous and Escherichia coli, being effective at low concentrations. Ns-AgNPs may be incorporated into wound dressings, surgical instruments, and medical devices to prevent infections and promote healing.