Stereological analysis of the brain in methamphetamine abusers compared to the controls

Abstract

Background: Methamphetamine (MA) is an addictive and stimulant drug that its abuse causes psychological and cognitive symptoms. MA abuse can cause structural changes in the brain. Objectives: The current study aimed to evaluate stereological changes of the brains of MA abusers, compared to the controls. Patients and Methods: The current case-control study was conducted on magnetic resonance (MR) images from MA abusers and healthy controls (n = 10 in each group), in Zahedan, Iran. The convenience sampling method was employed to select the subjects. MR images of the brains of the 2 groups in frontal, coronal, and sagittal axes with 4-mm slide thickness and 0.5-mm intervals were acquired. Parameters including total volume (V) and volume density (Vv) of different parts of the brain were estimated based on the Cavalieri point counting stereological method. To analyze the data, descriptive statistics and the Mann-Whitney U-test were applied. The significance level was considered P < 0.05. Results: The results showed that the volume of the cerebellum, the volume and volume density of the ventricles, and gray matter volume and volume density, and the basal ganglia volume density in MA group was significantly smaller than those of the controls (P < 0.05). But, white matter volume and volume density in the MA abusers were significantly larger than those of the healthy subjects (P < 0.05). In addition, there were no significant differences between the total brain, the hippocampus, and the basal ganglia volumes between the 2 groups. Conclusions: According to the results of the current study, MA abuse can cause structural changes in the brain components. MR imaging by the stereological methods can be employed as a technique to determine the level of such damages in substance abusers.