Perbedaan Kadar Trigliserida pada Sampel Plasma dan Serum Darah dengan Metode GPO PAP

Abstract

Triglycerides are very important for the human body because in the human body triglycerides are useful as a source of energy for the body in addition to other nutrients such as carbohydrates, protein, vitamins and minerals, but high triglyceride values are associated with the onset of heart attack, stroke and diabetes. To determine the level of triglycerides in the blood can use a sample, either serum or blood plasma. The use of serum samples can provide more appropriate results because they are not contaminated with the presence of anticoagulants used in plasma samples. The aim of this study was to determine whether there were differences in triglyceride levels in samples using serum and blood plasma. This research is a laboratory observational study, using a sample of 30 samples aged 19-25 years and using the GPO PAP method to determine the Triglyceride levels in the sample, then the data is analyzed using the t test to see whether or not there are differences in Triglyceride levels in serum and plasma samples. The results of this study were obtained that the average Triglyceride level in the plasma sample was 162.94 mg / dl, while in the serum sample was 228.50 mg / dL. Based on the t-test statistic, there is a significant difference between triglyceride levels using plasma and serum samples.